OsWRKY97, an Abiotic Stress-Induced Gene of Rice, Plays a Key Role in Drought Tolerance

Miaomiao Lv, Dejia Hou , Jiale Wan , Taozhi Ye , Lin Zhang, Jiangbo Fan, Chunliu Li, Yilun Dong , Wenqian Chen, Songhao Rong, Yihao Sun, Jinghong Xu, Liangjun Cai, Xiaoling Gao, Jianqing Zhu, Zhengjian Huang, Zhengjun Xu, Lihua Li

Plants，2023 Sep 21;12(18):3338（IF=4.5）

https://pubmed.ncbi.nlm.nih.gov/37765501/

Abstract

Drought stress is one of the major causes of crop losses. The WRKY families play important roles in the regulation of many plant processes, including drought stress response. However, the function of individual WRKY genes in plants is still under investigation. Here, we identified a new member of the WRKY families, OsWRKY97, and analyzed its role in stress resistance by using a series of transgenic plant lines. OsWRKY97 positively regulates drought tolerance in rice. OsWRKY97 was expressed in all examined tissues and could be induced by various abiotic stresses and abscisic acid (ABA). OsWRKY97-GFP was localized to the nucleus. Various abiotic stress-related cis-acting elements were observed in the promoters of OsWRKY97. The results of OsWRKY97-overexpressing plant analyses revealed that OsWRKY97 plays a positive role in drought stress tolerance. In addition, physiological analyses revealed that OsWRKY97 improves drought stress tolerance by improving the osmotic adjustment ability, oxidative stress tolerance, and water retention capacity of the plant. Furthermore, OsWRKY97-overexpressing plants also showed higher sensitivity to exogenous ABA compared with that of wild-type rice (WT). Overexpression of OsWRKY97 also affected the transcript levels of ABA-responsive genes and the accumulation of ABA. These results indicate that OsWRKY97 plays a crucial role in the response to drought stress and may possess high potential value in improving drought tolerance in rice.

Keywords: ABA; Oryza sativa L.; OsWRKY97; antioxidation; drought tolerance.