Genes Are Involved in the Retransfer of Zinc Ions during the Senescence of Zinc-Deficient Rice Leaves

Yangming Ma,Yanfang Wen,Cheng Wang,Ziniu Wu,Xiaojuan Yuan,Ying Xiong,Kairui Chen,Limei He,Yue Zhang,Zhonglin Wang,LeiLei Li,Zhiyuan Yang,Yongjian Sun,Zhongkui Chen,Jun Ma

International Journal of molecular sciences，2023 Sep 12;24(18):13989（IF=5.6）

https://doi.org/10.21203/rs.3.rs-3110004/v1

Abstract

Rice lacks sufficient amounts of zinc despite its vitality for human health. Leaf senescence enables redistribution of nutrients to other organs, yet Zn retransfer during deficiency is often overlooked. In this hydroponic experiment, we studied the effect of Zn deficiency on rice seedlings, focusing on the fourth leaf under control and deficient conditions. Growth phenotype analysis showed that the growth of rice nodal roots was inhibited in Zn deficiency, and the fourth leaf exhibited accelerated senescence and increased Zn ion transfer. Analyzing differentially expressed genes showed that Zn deficiency regulates more ZIP family genes involved in Zn ion retransfer. OsZIP3 upregulation under Zn-deficient conditions may not be induced by Zn deficiency, whereas OsZIP4 is only induced during Zn deficiency. Gene ontology enrichment analysis showed that Zn-deficient leaves mobilized more biological pathways (BPs) during aging, and the enrichment function differed from that of normal aging leaves. The most apparent "zinc ion transport" BP was stronger than that of normal senescence, possibly due to Zn-deficient leaves mobilizing large amounts of BP related to lipid metabolism during senescence. These results provide a basis for further functional analyses of genes and the study of trace element transfer during rice leaf senescence.

Keywords: ZIP; leaf senescence; rice; zinc deficiency; zinc ion retransfer.