Ectopic Expression of a Maize Gene ZmDUF1645 in Rice Increases Grain Length and Yield, but Reduces Drought Stress Tolerance

Yaqi Li, WeiWang , Changqiong Hu, Songjin Yang, Chuan Ma, JiachengWu, Yuwei Wang, Zhengjun Xu, Lihua Li, Zhengjian Huang, Jianqing Zhu, Xiaomei Jia, Xiaoying Ye, Zhiyuang Yang, Yongjian Sun, Huainian Liu and Rongjun Chen*

International Journal of Molecular Sciences（IF=5.924）2023, 24(12),9794

https://www.mdpi.com/1422-0067/24/12/9794/html

Abstract

As the human population grows rapidly, food shortages will become an even greater problem; therefore, increasing crop yield has become a focus of rice breeding programs. The maize gene, ZmDUF1645, encoding a putative member of the DUF1645 protein family with an unknown function, was transformed into rice. Phenotypic analysis showed that enhanced ZmDUF1645 expression significantly altered various traits in transgenic rice plants, including increased grain length, width, weight, and number per panicle, resulting in a significant increase in yield, but a decrease in rice tolerance to drought stress. qRT-PCR results showed that the expression of the related genes regulating meristem activity, such as MPKA, CDKA, a novel crop grain filling gene (GIF1), and GS3, was significantly changed in the ZmDUF1645-overexpression lines. Subcellular colocalization showed that ZmDUF1645 was primarily localized on cell membrane systems. Based on these findings, we speculate that ZmDUF1645, like the OsSGL gene in the same protein family, may regulate grain size and affect yield through the cytokinin signaling pathway. This research provides further knowledge and understanding of the unknown functions of the DUF1645 protein family and may serve as a reference for biological breeding engineering to increase maize crop yield.

Keywords: rice; maize; DUF1645 protein family; grain size; cytokinin