深刻揭示水稻抗稻瘟病基因PiZ-t和稻瘟病菌无毒基因AvrPiz-t的相互作用机制
时间： 2016-04-02 点击次数：次 作者：admin
Park CH, Shirsekar G, Bellizzi M, Chen S,Songkumarn P, Xie X, et al. (2016) The E3 Ligase APIP10 Connects the Effector AvrPiz-t to the NLR Receptor Piz-t in Rice. PLoS Pathog 12(3):e1005529. doi:10.1371/ journal.ppat. 1005529
Abstract
Although nucleotide-binding domain, leucine-rich repeat (NLR) proteins are the major immune receptors in plants, the mechanism that controls their activation and immune signaling remains elusive. Here, we report that the avirulence effector AvrPiz-t from Magnaporthe oryzae targets the rice E3 ligase APIP10 for degradation, but that APIP10, in return, ubiquitinates AvrPiz-t and thereby causes its degradation. Silencing of APIP10 in the non-Piz-t background compromises the basal defense against M. oryzae. Conversely, silencing of APIP10 in the Piz-t background causes cell death, significant accumulation of Piz-t, and enhanced resistance to M. oryzae, suggesting that APIP10 is a negative regulator of Piz-t. We show that APIP10 promotes degradation of Piz-t via the 26S proteasome system. Furthermore,we demonstrate that AvrPiz-t stabilizes Piz-t during M. oryzae infection. Together,our results show that APIP10 is a novel E3 ligase that functionally connects the fungal effector AvrPiz-t to its NLR receptor Piz-t in rice.