我所王静博士在The Plant Cell上发表论文揭示水稻株型建成的分子机制

3月14日，我校水稻研究所青年教师王静博士在植物领域里的顶级学术刊物《植物细胞》(The Plant Cell, 5年平均影响因子9.88)在线发表了题为Tissue-specific Ubiquitination by IPA1 INTERACTING PROTEIN 1 Modulates IPA1 Protein Levels to Regulate Plant Architecture in Rice的研究论文，揭示了水稻中一个E3泛素连接酶IPI1参与调控水稻株型建成的分子机制。

水稻株型发育是影响水稻产量的重要因素之一。IPA1是早期克隆得到的一个调控水稻理想株型建成的重要基因。本研究通过酵母双杂交的方法筛选得到了一个与IPA1互作的蛋白IPI1。IPI1编码了一个E3泛素连接酶，可以直接靶定IPA1并对其进行泛素化修饰。与以往植物中报道的E3泛素连接酶不同，IPI1对IPA1稳定性的影响在不同组织中由于作用机制的差异而截然不同。该项工作为进一步的解析E3泛素连接酶在植物中的作用机制提供了新的思路，同时IPI1基因对水稻产量的作用有望为后续育种工作提供新的材料和资源。

ipi1突变体和野生型水稻

A Proposed Model of IPI1 Mediated Post-Translational Modification of IPA1

王静博士2012年毕业于中国科学院遗传与发育生物学研究所，博士期间一直在李家洋院士实验室从事水稻理想株型基因IPA1的功能研究，相继在Nature Genetics, The Plant Cell等杂志上发表论文。目前她在我校水稻研究所陈学伟教授实验室从事水稻抗病机制研究，近三年来以第一作者或共同第一作者先后在The Plant Cell, Plant Cell and Environment等国际期刊发表SCI论文7篇，累计影响因子近30。

王静博士工作照

王静博士

论文链接：http://www.plantcell.org/content/early/2017/03/14/tpc.16.00879.abstract

论文摘要：Plant architecture, a collection of genetically controlled agronomic traits, is one of the decisive factors that determine grain production. IDEAL PLANT ARCHITECTURE 1 (IPA1) encodes a key transcription factor having pleiotropic effects on regulating plant architecture in rice (Oryza sativa), and IPA1 expression is controlled at the post-transcriptional level by microRNA156 and microRNA529. Here, we report the identification and characterization of IPA1 INTERACTING PROTEIN 1 (IPI1), a RING-finger E3 ligase that can interact with IPA1 in the nucleus. IPI1 promotes the degradation of IPA1 in panicles while it stabilizes IPA1 in shoot apexes. Consistent with these findings, the ipi1 loss-of-function mutants showed markedly altered plant architecture, including more tillers, enlarged panicles, and increased yield per plant. Moreover, IPI1 could ubiquitinate IPA1-mediated complex with different polyubiquitin chains, adding K48-linked polyubiquitin chains in panicles and K63-linked polyubiquitin chains in the shoot apex. These results demonstrate that IPI1 affects plant architecture through precisely tuning IPA1 protein levels in different tissues in rice, and provide new insight into the tissue-specific regulation of plant architecture and important genetic resources for molecular breeding applications.