DGW1, encoding an hnRNP-like RNA binding protein, positively regulates grain size and weight by interacting with GW6 mRNA

Lingfeng Li , Jijin Li, Keke Liu, Chenglong Jiang, Wenhu Jin, Jiangkun Ye, Tierui Qin, Binjiu Luo, Zeyu Chen, Jinzhao Li, Fuxiang Lv, Xiaojun Li , Haipeng Wang , Jinghua Jin , Qiming Deng , Shiquan Wang , Jun Zhu , Ting Zou , Huainian Liu , Shuangcheng Li , Ping Li , Yueyang Liang

Plant Biotechnol J. 2024 Feb;22(2):512-526. ( IF=13.8)

https://pubmed.ncbi.nlm.nih.gov/37862261/

Abstract

Grain size and weight determine rice yield. Although numerous genes and pathways involved in regulating grain size have been identified, our knowledge of post-transcriptional control of grain size remains elusive. In this study, we characterize a rice mutant, decreased grain width and weight 1 (dgw1), which produces small grains. We show that DGW1 encodes a member of the heterogeneous nuclear ribonucleoprotein (hnRNP) family protein and preferentially expresses in developing panicles, positively regulating grain size by promoting cell expansion in spikelet hulls. Overexpression of DGW1 increases grain weight and grain numbers, leading to a significant rise in rice grain yield. We further demonstrate that DGW1 functions in grain size regulation by directly binding to the mRNA of Grain Width 6 (GW6), a critical grain size regulator in rice. Overexpression of GW6 restored the grain size phenotype of DGW1-knockout plants. DGW1 interacts with two oligouridylate binding proteins (OsUBP1a and OsUBP1b), which also bind the GW6 mRNA. In addition, the second RRM domain of DGW1 is indispensable for its mediated protein-RNA and protein-protein interactions. In summary, our findings identify a new regulatory module of DGW1-GW6 that regulates rice grain size and weight, providing important insights into the function of hnRNP-like proteins in the regulation of grain size.

Keywords: DGW1; GW6; RNA-binding protein; grain size; rice.