OsSHOC1 and OsPTD1 are essential for crossover formation during rice meiosis

Yun Ren, Dan Chen, Wenjie Li, Dan Zhou, Tao Luo, Guoqiang Yuan, Jing Zeng, Ye Cao, Zhongshan He, Ting Zou, Qiming Deng, Shiquan Wang, Aiping Zheng, Jun Zhu, Yueyang Liang, Huainian Liu, Lingxia Wang, Ping Li*,  Shuangcheng Li*

The Plant Journal（IF=5.775，TOP），2019，98：315-328

https://onlinelibrary.wiley.com/doi/pdf/10.1111/tpj.14214

Abstract

Meiosis is essential for eukaryotic sexual reproduction and plant fertility, and crossovers (COs) are essential for meiosis and the formation of new allelic combinations in gametes. In this study, we report the isolation of a meiotic gene, OsSHOC1, and the identification of its partner, OsPTD1. Osshoc1 was sterile both in male and female gametophytes, and it showed a striking reduction in the number of meiotic COs, indicating that OsSHOC1 was required for normal CO formation. Further investigations showed that OsSHOC1 physically interacted with OsPTD1 and that the latter was also required for normal CO formation and plant fertility. Additionally, the expression profiles of both genes were consistent with their functions. Our results suggest that OsSHOC1 and OsPTD1 are essential for rice fertility and CO formation, possibly by stabilizing the recombinant intermediates during meiosis.